分子量:34.3 kDa

纯度:>98% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human IL-17 RA, His Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measured by its ability to inhibit IL-17 induced IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells.
The ED50 for this effect is typically 0.01-0.1 g/mL in the presence of 10 ng/mL recombinant human IL-17.
Measured by its binding ability in a functional ELISA. Immobilized Human IL17 /IL17A,Tag Free (Catalog # ILA-H5118) at 2 μg/mL (100 μL/well ) can bind Human IL-17 RA, His Tag (Catalog # ILA-H5222) with a linear ranger of 0.3 - 10 ng/mL.

产品特性:Human IL-17 RA, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 34.3 kDa. The predicted N-terminus is Leu 33. The reducing (R) protein migrates as 55-60 kDa in SDS-PAGE  due to glycosylation.

产品背景:Interleukin 17 receptor A (IL17RA) is also known as cluster of differentiation w217 (CDw217), is a pro-inflammatory cytokine secreted by activated T-lymphocytes, belong to ubiquitous type I membrane glycoprotein, and binds with low affinity to interleukin 17A (IL17A). IL 17R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested . IL 17RA associates with IL 17RC to form a signaling receptor complex for IL 17 and IL 17F . Ligand and IL 17RA ligation promotes T cell activation and the production of IL - 6, G-CSF, SCF, and multiple pro-inflammatory chemokines. Defects in IL17RA are the cause of familial candidiasis type 5 (CANDF5).