分子量:16.5 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human Niemann-Pick Type C2, His Tag by the LAL method.

Buffer:PBS, pH 7.4

生物活性:

产品特性:Human Niemann-Pick Type C2, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 16.5 kDa. The predicted N-terminus is Glu 20. The reducing (R) protein migrates as 20-24 kDa in SDS-PAGE  due to glycosylation.

产品背景:Niemann-Pick disease type C2 protein (NPC2) is also known as epididymal secretory protein E1 and human epididymis-specific protein 1 (HE1). NPC2 is a intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. Also,NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Furthermore,the secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport.